Quantitative Analysis of VWF Multimer Structure. . Vwf Multimer Analysis Essay

When is Willebrand factor (vWF) multimer analysis indicated?

The analysis of VWF multimers generally consists of four steps: (1) electrophoresis of plasma in an agarose gel, (2) either gel fixation or transfer of the electrophoretic protein product to a membrane, (3) immunodetection of the protein, and (4) evaluation of the protein in the gel or membrane.Von Willebrand factor (VWF) is a multimeric glycoprotein ranging in size from small to ultra-large molecular weight forms of up to 20 x 10 6 Daltons (Da). It is synthesised in: 1. The vascular endothelial cells where it is stored in the Weibel-Palade bodies prior to release and.Derivatization was followed by detailed structural and functional examination including multimer analysis (MMA) and Fourier transform infrared spectroscopy (FTIR). Functional differences between the NEM-derivatized sample and the control sample were detected using an in vitro flow chamber system with respect to VWF-mediated platelet adhesion to collagen.

Phenotypic characterization comprised plasma VWF parameters and multimer analysis using low- and intermediate-resolution gels combined with an optimized visualization system. VWF genotyping was performed in all index cases (ICs). Results: Abnormal multimers were present in 57 out of 150 ICs; however, only 29 out of these 57 (51%) had VWF.Appendix I: Multimeric analysis of Von Willebrand factor and classification of Von Willebrand. 1997-07-01 00:00:00 Determination of the Von Willebrand factor (VWF) multimer composition in plasma and platelets is important in the diagnosis and classification of subtypes of Von Willebrand disease (VWD). The multimeric distribution of both plasma or platelet V W F can be determined by gel.

VWF quantity or its functions mediating platelet adhesion and aggregation at sites of vascular damage and carrying pro-coagulant factor VIII (FVIII). Phenotype and genotype analysis in patients with the three VWD types has aided understanding of VWF structure and function.

VWF is a multimeric, high molecular weight glycopro- tein that plays a pivotal role, under high shear conditions, in platelet adhesion and aggregation, and in stabilizing blood coagulation factor VIII (FVIII).

Laboratory Use of VWF multimer assay recommended only when initial VWD testing identifies an abnormal result, or clinical information suggests a high likelihood of abnormal VWF multimer analysis (Mayo Medical Laboratories).

In patients with abnormal results, a VWF multimer analysis may help with classification, especially when the activity is reduced relative to the antigen. Comparison of VWF antigen (VWF:Ag), VWF ristocetin cofactor activity (VWF:RCo), and VWF collagen binding activity (VWF:CB) may provide similar information.

The described procedures of vWF multimer analysis with high-sensitivity Cy5 fluorescence detection and direct comparison of high- and low-resolving gels for screening and detection of the complete range of high- and low-molecular vWF multimers is efficient and useful for screening, detecting, and classifying vWD subtypes and makes this method diagnostically and clinically relevant.

Analysis of r-vWF by multimer analysis revealed a multimer pattern equal in number of high molecular weight multimer to pd-vWF, but absence of satellite bands. Two-dimensional electrophoretic analysis of both the primary dimer and the complete multimer pattern of r-vWF showed that the recombinant coagulation factor was composed exclusively of intact and mature subunits.

To assess VWF:Lx diagnostic accuracy, 492 patient plasma samples, including 40 previously characterized VWD patient samples, were tested for VWF antigen (VWF:Ag) and VWF:RCo by either aggregometry or flow cytometry, and VWF:Lx with supplemental VWF multimer analysis when indicated.

Von Willebrand Factor (VWF) Assays: Introduction.

When is Willebrand factor (vWF) multimer analysis indicated?

Von Willebrand Factor Multimeric Analysis - Coagulation.

Characterization of Von Willebrand Factor Multimer.